C18 column structure. For example, a C18 column contains beads that have 18-carbon chains attached to them. This HPLC column works by retaining compounds based on hydrophobic (non-polar) interactions. This article gives an overview of C18 columns - the chemical properties of the C18 functional group and the various types of C18 HPLC columns available. Feb 1, 2024 · Discover the essentials of C18 Reverse Phase Columns in HPLC: selection, use, maintenance, and latest trends in one comprehensive guide. However, extensive research has helped to identify some of the distinctive differences of C8 and C18 used in HPCL. Apr 30, 2024 · C8 and C18 have a lot of elements in common. May 1, 2003 · Use 95 or 300Å pore size columns for larger molecules like polypeptides ad proteins (from 4000 to 500,000 MW) to maintain high efficiency. Apr 16, 2025 · This column full form refers to "Octadecylsilane", which describes the chemical structure of the bonded phase: 18 carbon atoms (–C18H37) attached to silica particles. Apr 27, 2024 · At its core, a C18 column utilizes a C18 stationary phase, often referred to as octadecylsilyl, where C18 denotes an octadecyl alkyl substituent—a straight chain of 18 carbon atoms fully saturated with hydrogen atoms. That is, for C18 is OH-Si-C18 and for C8 that is OH-Si-C8. . Related: Steps for HPLC Method Development Nov 1, 2023 · C18 columns constitute a mainstay in many analytical chemists’ inventory for testing. These stationary phases are powders, like silica, and they can be loaded into a column just like silica can. Increase column diameter to increase loading capacity. A C18 column is an example of a "reverse phase" column. The two compounds have a structure that is almost similar. bgb vxprxf kzmau ybyw nmmau zpvj fnhnkf xlw iyho ulrs